IL-6 and IL-8 secretion by human glioma cells proliferating after Gamma-knife irradiation

One of the modern methods treating patients with primary and recurrent brain tumors is radiosurgical irradiation using Gamma Knife, which allows therapeutic doses to be delivered not exceeding 2.5 cm in diameter 1–2 sessions. Tumor cells on periphery this tissue volume that receive lower radiation can resume proliferation serve as a source recurrence. The increase dose may cause necroses formation worsening prognosis. properties glioblastoma survive long after stereotactic are still poorly known. aim work was evaluate expression IL-6 IL-8 by A172, R1, T2, T98G cell lines resumed sublethal Knife irradiation. Cells were irradiated once at ranging from 6 16 Gy, then cultured for 40 days. Cell number counted weekly; lethal each line determined. In cultures descendant single most resistant cells, level secretion 96 hours cultivation (ng/1000 cells) determined ELISA. all four secreted into culture medium. highest production cytokines, never before demonstrated glioblastomas, discovered R1 cells. Glioblastoma T2 also had high interleukin levels. contrast these lines, A172 (highly sensitive action cytostatic drugs radiation) 30 times than exhibited low interleukins level. increased and, lesser extent, IL-8. dependence cytokine linear. contrast, reduced under multidirectional changes different long-term persisted more month. presented results cast doubt possibility use potential biomarkers early diagnosis, therapy monitoring well prognostic markers disease course.